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KMID : 0350519950480020379
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Journal of Catholic Medical College
1995 Volume.48 No. 2 p.379 ~ p.394
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Relationship between Quantitation of Virus RNA and Its Genotype and Regional Specific Antibody Responses in Korean Patients with Chronic HCV Infection
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Abstract
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Quantitation of HCV RNA by competitive polymerase chain reation (PCR) has been used to investigate the factors that influence diseas severity and therapeutic effectiveness. However, the relationships between HCV replication, and genotypes, immune
responses, disease stages and responses to the interferon treatment have not yet been clarified.
This study was conducted to define the correlation between the serum HCV RNA levels, and HCV genotypes and antibody responses to HCV core (C22-3), NS3 (C33C) and NS4 (5-1-1, C100-3) proteins.
Sera from forty-five patiens with chronic HCV infection (33 chronic hepatitis, 12 hepatocellular carcinoma) who were positive for anti-HCV were tested for antibody responses to regional specific HCV proteins by a second generation recombinant
immunoblot
assay (RIBA-2), and for HCV RNA detection, HCV genotyping and quantitation of HCV RNA by reverse transcription (RT)-nested PCR.
@ES The results were as follows :
@EN 1. Forty-one of the 45 patients (9.11%) had antibodies to HCV C22-3 and C33C proteins irrespective of the serum HCV RNA levels.
2. No significant differences were seen in the HCV RNA levels between chronic hepatitis and hepatocellular carcinoma, but antibody response to HCV NS4 protein was higher in chronic hepatitis than in hepatocellular carcinoma (P<0.05).
3. The levels of the antibodies' reactivity of HCV core and NS3 proteins correlated significantly with the concentration of the serum HCV RNA (r=0.36, P<0.05; r=0.33, P<0.05, respectively).
4. More than one antibody to the four antigens in RIBA-2 were detected in 31 of the 45 patients(68.9%) in the group with high viremic levels of≥10E6 copies/ml of serum, and in 14 of 45 patients(31.1%) in the group with low viremic levels of
<10E6
copies/ml serum.
5. Antibodies ot HCV NS4 (5-1-1 and C100-3) protein were detected less frequently than those to HCV core and NS3 proteins, but not to a statistically significant extent.
6. No significant differences were seen in HCV RNA levels between HCV-J (1b) and HCV-K2a (2a) type, but detection rates of antibodies to HCV NS3 and-NS4 proteins were higher in HCV-J (1b) type than in HCV-K2a (2a) type (P<0.05).
These results suggest that low viremic states may have poor antibody responses to HCV protein compared to high viremic states, and HCV core and NS3 protein may be more immunogenic than HCV NS4 protein, and HCV geotypes may influence the immune
responses
of antiboty to HCV protein regardless of HCV replication.
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KEYWORD
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